Composite
Part:BBa_K098993:Design
Designed by: Harvard iGEM 08 Group: iGEM08_Harvard (2008-10-26)
heat sensitive cI QPI with low promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is a mutagenized form because the original product had an extra PstI site between the repressor and terminator. While there are still extra basepairs between the end of the repressor sequence and the Biobricks scar, they no longer code for a PstI site.
The extra base pairs are: [repressor coding region] CTCCAGCGGCCGC [Biobricks scar]
Source
PGW7 plasmid and various BioBricks parts.